Immunoblotting on polyvinyldifluoride improves detection of oligoclonal IgG bands in CSF.
نویسندگان
چکیده
منابع مشابه
Optimizing detection of oligoclonal bands in cerebrospinal fluid by use of isoelectric focusing with IgG immunoblotting.
Multiple sclerosis (MS) is difficult to diagnose using clinical evidence alone, especially early in the course of the disease. Yet, the importance of early detection is increasing because immunomodulatory therapy is believed to be most beneficial early in the disease process.1 MS is first suspected when recurrent clinical symptoms point to local destruction of myelin in at least 2 anatomic loca...
متن کاملCSF -- oligoclonal bands in multiple sclerosis.
The present paper is concerned with a correlation between clinical parameters of multiple sclerosis and different agar gel electrophoretical patterns in CSF. The agar gel electrophoresis represents clearly the most sensitive method for the demonstration of oligoclonal bands in CSF.
متن کاملCorrelation of the IgG index and oligoclonal bands in the CSF of patients with multiple sclerosis
Klíčová slova oligoklonální pásy – mozkomíšní mok – IgG index – roztroušená skleróza Abstract Objective: The aim of this study was to assess the correlation between IgG index values and the number of the oligoclonal IgG bands (OCB) in the cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS). Material and Methods: The set of 150 patients consisted of 41 males and 109 females (aged ...
متن کاملDetection of oligoclonal IgG bands in cerebrospinal fluid by isoelectric focusing on thin layer agarose gels.
The detection of oligoclonal bands of IgG in cerebrospinal fluid by isoelectric focusing is a useful aid in the diagnosis of multiple sclerosis. At present, this test is performed on polyacrylamide gels which are technically very difficult to prepare and are not suitable for the routine laboratory. A technique is described for isoelectric focusing on thin layer agarose gels which permits oligoc...
متن کاملCerebrospinal fluid oligoclonal bands in the diagnosis of multiple sclerosis. Isoelectric focusing with IgG immunoblotting compared with high-resolution agarose gel electrophoresis and cerebrospinal fluid IgG index.
Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. We compared the diagnostic performance of isoelectric focusing (IEF) combined with IgG immunoblotting to high-resolution agarose gel electrophoresis for the detection of intrathecal synthesis of IgG due to the MS disease process. In 20 patients with definite MS, IEF and high-resolution agarose gel el...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1987
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/33.9.1669