Immunization trials with an avian chlamydial MOMP gene recombinant adenovirus

Oral immunization with chlamydial major outer membrane protein (MOMP).

The effect of vaccination to stimulate mucosal immunity with a purified subunit vaccine of chlamydial major outer membrane protein (MOMP) on subsequent ocular challenge with Chlamydia trachomatis was studied in cynomolgus monkeys. Monkeys were immunized with MOMP by intraperitoneal priming plus oral boosting, with or without ocular boosting, or by ocular immunization alone. Cholera toxin was us...

Production of Recombinant Adenovirus Containing Human Interlukin-4 Gene

Objective(s) Recombinant adenoviruses are currently used for a variety of purposes, including in vitro gene transfer, in vivo vaccination, and gene therapy. Ability to infect many cell types, high efficiency in gene transfer, entering both dividing and non dividing cells, and growing to high titers make this virus a good choice for using in various experiments. In the present experiment, a reco...

Induction of Tumor-Protective Immunity and Adenovirus Encoding a Lymphoma Idiotype: Immunization with a Recombinant

production of recombinant adenovirus containing human interlukin-4 gene

objective(s) recombinant adenoviruses are currently used for a variety of purposes, including in vitro gene transfer, in vivo vaccination, and gene therapy. ability to infect many cell types, high efficiency in gene transfer, entering both dividing and non dividing cells, and growing to high titers make this virus a good choice for using in various experiments. in the present experiment, a reco...

Rapid functional exhaustion and deletion of CTL following immunization with recombinant adenovirus.

Replication-deficient adenoviruses (recombinant adenovirus (rec-AdV)) expressing different transgenes are widely used vectors for gene therapy and vaccination. In this study, we describe the tolerization of transgene-specific CTL following administration of beta-galactosidase (beta gal)-recombinant adenovirus (Ad-LacZ). Using MHC class I tetramers to track beta gal-specific CTL, we found that a...