Immunization of Cattle with Live Tachyzoites of Neospora caninum Confers Protection against Fetal Death

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Immunization of mice with plasmid DNA coding for NcGRA7 or NcsHSP33 confers partial protection against vertical transmission of Neospora caninum.

The purpose of the present study was to use direct plasmid deoxyribonucleic acid (DNA) injection to identify specific antigens that confer protection against congenital transfer of Neospora caninum. Inbred BALB/c mice were vaccinated before pregnancy with a recombinant plasmid containing sequences encoding N. caninum antigen NcGRA7 or NcsHSP33. The mice were challenged with N. caninum tachyzoit...

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Susceptibility of various cell lines to Neospora caninum tachyzoites cultivation

Neospora caninum is a coccidian protozoan parasite which is a major cause of bovine abortions and neonatal mortality in cattle, sheep, goat and horse. Occasionally, cultured cells are used for isolation and multiplication of the agent in vitro with several purposes. In this study the tachyzoite yields of N. caninum were compared in various cell cultures as the host cell lines. Among the cell cu...

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Seminested PCR for Diagnosis of Neospora caninum Infection in Cattle.

Neospora caninum, an apicomplexan protozoan, is regarded as a major cause of abortion and stillbirth in cattle in countries world-wide. The ability to detection N.caninum in tissue samples can be a useful detection diagnostic tool for use in the study of the pathogenicity, immunoprophylaxis, and treatment of Neospora infection. However, molecular biology is one of the most sensitive tools for d...

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Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against experimental Neospora caninum infection.

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 2007

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.00777-06