Immune responses in COVID-19 patients during breakthrough infection with SARS-CoV-2 variants Delta, Omicron-BA.1 and Omicron-BA.5

نویسندگان

چکیده

Background Breakthrough infections with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants are increasingly observed in vaccinated individuals. Immune responses towards SARS-CoV-2 variants, particularly Omicron-BA.5, poorly understood. We investigated the humoral and cellular immune of hospitalized COVID-19 patients during Delta Omicron infection waves. Methods The corresponding variant respective were identified by whole genome sequencing. Humoral analyzed ELISA a cell culture-based neutralization assay against D614G isolate (wildtype), Alpha, (AY.43) (BA.1 BA.5). Cellular immunity was evaluated an IFN-γ ELISpot assay. Results On level, showed minor response after stimulating PBMCs mutated regions variants. Neutralizing antibody titers Omicron-BA.1 especially BA.5 strongly reduced. Double-vaccinated breakthrough significantly increased neutralizing compared to double-vaccinated uninfected controls (median complete titer (NT 100 ) 640 versus 80, p<0.05). BA.1 NT 160 20 controls, p=0.07) that received booster vaccination 50 p=0.68). For boosted infection, we found no enhancing effect on Conclusion reduced infections. but not Omicron-BA.5 vaccination. Despite those may still be vulnerable for reinfections or other newly emerging concern.

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ژورنال

عنوان ژورنال: Frontiers in Immunology

سال: 2023

ISSN: ['1664-3224']

DOI: https://doi.org/10.3389/fimmu.2023.1150667