Immobilization of Pectinolytic Enzymes on Nylon 6/6 Carriers

Pectinolytic enzymes are an important tool for sustainable food production, with a wide range of applications in processing technologies as well the extraction bioactive compounds from pectin-rich raw materials. In present study, we immobilized commercial pectinase preparation onto pellet and thread shaped nylon 6/6 carriers assessed its stability reusability. Five preparations were tested different pectin de-polymerizing activities (pectinase, polygalacturonase, lyase activities). Thereafter, Pectinex® Ultra Tropical preparation, exhibiting highest catalytic among studied (p < 0.0001), was on using dimethyl sulfate glutaraldehyde. The immobilization yield accordance carrier surface area available enzyme attachment, it 1.25 ± 0.10 U/g threads, which over 40 times higher than that pellets. However, inactivation not dependent shape carrier, indicating attachment similar. half-life fast phase at 4 °C 12.8 days. After 5 weeks, unused threads retained 63% their initial activity. Reusability study showed after 20 successive cycles remaining activity 22%, good prospects reusability industrial application.