Immobilization of nitrate reductase within polyacrylamide gels

Analysis of nitrate reductase mRNA expression and nitrate reductase activity in response to nitrogen supply

Nitrate is one of the major sources of nitrogen for the growth of plants. It is taken up by plant roots and transported to the leaves where it is reduced to nitrite in the. The main objective of this research was to investigate stimulatory effects of sodium nitrate, potassium nitrate, ammonia and urea on the production/generation of the nitrate reductase mRNA in Triticum aestivum plants. The pl...

Formation and Structure of Polyacrylamide Gels

Acrylamide and N,N-methylenebis(acry1amide) (AAm-Bis) copolymerization has been investigated in water at a monomer concentration of 1.8 w/v %. Conversion of monomer and pendant vinyl groups was measured as a function of the reaction time up to the onset of macrogelation. Experimental results indicate that 80% of pendant vinyl groups are consumed by cyclization reactions. When the monomer concen...

Demonstration of rhodanese activity in polyacrylamide gels.

Rhodanese activity from crude extracts of Thiobacillus sp. strain IV-85 was demonstrated in polyacrylamide gels after incubation in the reaction mixture by staining with dichloroindophenol in the presence of methylphenazonium methosulfate. The sensitivity of the staining system was found to be 8 x 10 moles of sulfite.

Synthesis and Characteristics of Mesoporous Sol-gels for Lipase Immobilization

Enzyme cost is the major problem for industrial scale application. Immobilization is a promising approach to moderate the enzyme cost factor and increase its stability and activity. In this study, sol-gel method was used to prepare the immobilization platform and entrapped lipase as one of the most used enzyme in dairy processing, cosmetics and pharmaceutical industries. Lipase from Candida rug...

Steps toward peptide polymerization in polyacrylamide gels