Immobilization of Antioxidant Enzyme Catalase on Porous Hybrid Microparticles of Vaterite with Mucin

Catalase is one of the crucial antioxidant enzymes with diverse applications in textile, food industries, wastewater treatment, cosmetics, and pharmaceutics, which, however, highly sensitive to environmental challenges. Resisting loss activity prolongation formulation storage can be achieved via catalase entrapment into insoluble carriers. Affordable degradable vaterite proposed as amicable material for immobilization. To improve carrier properties vaterite, it was co-precipitated mucin from pig's stomach producing ca 5 ?m hybrid mucin/vaterite microparticles. impregnated crystals by means adsorption without chemical modifications. The presence matrix partially hinders penetration reduces capacity (for 0.1 mg mL?1 catalase, 2.3 vs 1.5 g?1 pristine microparticles, respectively) but significantly promotes protection upon under action temperature, organic solvent (acetonitrile), proteolytic enzyme (trypsin). Hybrid microcrystals are pH-sensitive better retain at pH 3–5 due catalase-mucin complexation. Immobilized used 5–6 consecutive cycles until loses catalytic activity. Altogether, these findings indicate promises microparticles immobilization enzymes.