Imaging promoter activity to monitor gene expression using Intracellular Multiaptamer Genetic tag (IMAGEtag)

Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters

We describe a ribonucleic acid (RNA) reporter system for live-cell imaging of gene expression to detect changes in polymerase II activity on individual promoters in individual cells. The reporters use strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags) that can be expressed from a promoter of choice. For imaging, the cells are incubated with their ligands...

Live-cell Imaging of Pol II Promoter Activity to Monitor Gene Expression with RNA reporters

Genetic Variation of the IL-28B Promoter Affecting Gene Expression

The current standard of care for the treatment of chronic hepatitis C is pegylated interferon-α (PEG-IFNα) and ribavirin (RBV). The treatment achieves a sustained viral clearance in only approximately 50% of patients. Recent whole genome association studies revealed that single nucleotide polymorphisms (SNPs) around IL-28B have been associated with response to the standard therapy and could pre...

Using Branch Predictors to Monitor Brain Activity

A key problem with neuroprostheses and brain monitoring interfaces is that they need extreme energy efficiency. One way of lowering energy is to use the low power modes available on the processors embedded in these devices. We present a technique to predict when neuronal activity of interest is likely to occur, so that the processor can run at nominal operating frequency at those times, and be ...

LuxCDE-luxAB-based promoter reporter system to monitor the Yersinia enterocolitica O:3 gene expression in vivo

It is crucial to understand the in vitro and in vivo regulation of the virulence factor genes of bacterial pathogens. In this study, we describe the construction of a versatile reporter system for Yersinia enterocolitica serotype O:3 (YeO3) based on the luxCDABE operon. In strain YeO3-luxCDE we integrated the luciferase substrate biosynthetic genes, luxCDE, into the genome of the bacterium so t...