Imaging Collagen in Scar Tissue: Developments in Second Harmonic Generation Microscopy for Biomedical Applications
نویسندگان
چکیده
منابع مشابه
Imaging Collagen in Scar Tissue: Developments in Second Harmonic Generation Microscopy for Biomedical Applications
The ability to respond to injury with tissue repair is a fundamental property of all multicellular organisms. The extracellular matrix (ECM), composed of fibrillar collagens as well as a number of other components is dis-regulated during repair in many organs. In many tissues, scaring results when the balance is lost between ECM synthesis and degradation. Investigating what disrupts this balanc...
متن کاملMultiphoton fluorescence and second-harmonic-generation microscopy for imaging structural alterations in corneal scar tissue in penetrating full-thickness wound.
The transparency of the cornea relies on the special spatial arrangement of stromal collagen. Any pathological abnormality that leads to the wound healing responses and consequential disruption of collagen alignment may hinder corneal transparency. Understanding wound healing may be crucial for the successful applications of clinical procedures such as refractive surgery. Therefore, the develop...
متن کاملSecond-harmonic generation imaging of collagen in ancient bone
Second-harmonic generation imaging (SHG) captures triple helical collagen molecules near tissue surfaces. Biomedical research routinely utilizes various imaging software packages to quantify SHG signals for collagen content and distribution estimates in modern tissue samples including bone. For the first time using SHG, samples of modern, medieval, and ice age bones were imaged to test the appl...
متن کاملSecond-harmonic imaging of collagen.
Molecules that have no center of symmetry are able to convert light to its second harmonic, at twice the frequency and half the wavelength. This only happens with any efficiency at very high light intensities such as are given by a pulsed laser, and because the efficiency of the process depends on the square of the intensity, it will be focal plane selective in exactly the same way as two-photo...
متن کاملEstimation of helical angles of myosin and collagen by second harmonic generation imaging microscopy.
We performed Second Harmonic Generation (SHG) imaging microscopy of endogeneous myosin-rich and collagen-rich tissues in amphibian and mammals. We determined the relative components of the macroscopic susceptibility tensor chi((2)) from polarization dependence of SHG intensity. The effective orientation angle theta(e) of the harmonophores has been determined for each protein. For myosin we foun...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2017
ISSN: 1422-0067
DOI: 10.3390/ijms18081772