Imaging and differentiation of mouse embryo tissues by ToF-SIMS
نویسندگان
چکیده
منابع مشابه
Spatiotemporal lipid profiling during early embryo development of Xenopus laevis using dynamic ToF-SIMS imaging.
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging has been used for the direct analysis of single intact Xenopus laevis embryo surfaces, locating multiple lipids during fertilization and the early embryo development stages with subcellular lateral resolution (∼4 μm). The method avoids the complicated sample preparation for lipid analysis of the embryos, which requires selective ...
متن کاملC60-ToF SIMS imaging of frozen hydrated HeLa cells.
Sample preparation continues to be a major challenge for secondary ion mass spectrometry studies of biological materials. Maintaining the native hydrated state of the material is important for preserving both chemical and spatial information. Here, we discuss a method which combines a sample wash and dry protocol discussed by Berman et al1 (1) followed by plunge freezing in liquid ethane for a ...
متن کاملToF-SIMS imaging mass spectrometry of microbial systems
Organic biomarkers may greatly aid in the interpretation of geobiological systems, but their significance is often restricted by the loss of spatial information during conventional sample work-up (solvent extraction). Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) is a quasi-non-destructive technique that simultaneously detects inorganic and organic molecules on solid surfaces, witho...
متن کاملTOF-SIMS in Cosmochemistry
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) was introduced into cosmochemistry about a decade ago. Major advantages of TOF-SIMS compared to other ion microprobe techniques are (a) parallel detection of all secondary ions with one polarity in a single measurement – both polarities in subsequent analyses, (b) high lateral resolution, (c) sufficient mass resolution for separation of ...
متن کاملSurface Characterization of Polymer Blends by XPS and ToF-SIMS
The surface properties of polymer blends are important for many industrial applications. The physical and chemical properties at the surface of polymer blends can be drastically different from those in the bulk due to the surface segregation of the low surface energy component. X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary mass spectrometry (ToF-SIMS) have been widely used...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Mass Spectrometry
سال: 2007
ISSN: 1387-3806
DOI: 10.1016/j.ijms.2006.09.029