<i>Lactobacillus plantarum</i> AR495 improves stress-induced irritable bowel syndrome in rats by targeting gut microbiota and Mast cell-PAR2-TRPV1 signaling pathway

Probiotics have great potential in regulating intestinal pain. In this study, the effects of Lactobacillus plantarum AR495 on visceral sensitivity and gut microbiota irritable bowel syndrome (IBS) rats were studied. The results showed that tryptase released after mast cell activation degranulation plays a key role pain, L. reduced stimulation colonic cells expression protease-activated receptor 2 (PAR2) TRPV1 dorsal root ganglia (DRG). Research further supplementation with increased level short-chain fatty acids (SCFAs) enhanced barrier function colon. addition, analysis colon indicated promoted proliferation Bifidobacterium inhibited Lachnospiraceae, which alleviated imbalance caused by IBS to certain extent. total, might reduce through Mast cell-PAR2-TRPV1 signaling pathway maintaining homeostasis barrier.