<i>In vivo</i> phage display: identification of organ-specific peptides using deep sequencing and differential profiling across tissues

Abstract In vivo phage display is widely used for identification of organ- or disease-specific homing peptides. However, the current in biopanning approaches fail to assess biodistribution specific peptide phages across tissues during screen, thus necessitating laborious and time-consuming post-screening validation studies on individual phages. Here, we adopted bioinformatics tools RNA sequencing analysis high-throughput (HTS) data estimate representation peptides vivo. The from screen were analyzed using differential binding—relative each target organ versus a panel control organs. Application this approach model study low-diversity T7 library with spiked-in brain demonstrated brain-specific binding resulted novel lung- Our provides broadly applicable streamline increase its reproducibility success rate.