منابع مشابه
Analysis of one-step and two-step real-time RT-PCR using SuperScript III.
Real-time reverse transcription polymerase chain reaction (RT-PCR) is a commonly used technique to analyze gene expression. There has been little research conducted to test if SuperScript III quantitative one-step (reverse transcription carried out in the same tube as PCR) and two-step (reverse transcription carried out in a separate reaction) RT-PCR systems provide similar real-time results. I...
متن کاملTwo-Step Extraction of the Lower First Molar for Class III Treatment in Adult Patient
The aim of this article is to describe a case report of Class III malocclusion treatment with lower first molar extraction. The 27-year-old Caucasian male patient presented a symmetric face with a straight profile, hyperdivergent growth pattern, molar and cuspid Class III relation, and an anterior crossbite as well as a mild crowding on cuspids area, in both upper and lower arches and a tendenc...
متن کاملA neutral Fe(III) compound exhibiting a two-step spin transition and dielectric anomalies.
The mononuclear ferric compound Fe(H-5-Cl-thsa-Me)(5-Cl-thsa-Me)·H2O () was synthesized and characterized using powder/single-crystal X-ray diffraction, Mössbauer spectroscopy, differential scanning calorimetry (DSC), and magnetic susceptibility measurements. This photo-responsive compound shows reversible, two-step spin-crossover behaviour. Moreover, dielectric anomalies were observed during t...
متن کاملTwo-step, PCR-free telomerase detection by using exonuclease III-aided target recycling.
We report the sensitive detection of telomerase activity by using exonuclease III-aided target recycling to amplify the signal produced by a chimeric LNA-DNA molecular beacon. We demonstrate the specific detection of as few as 30 telomerase-positive breast cancer cells in a single-measurement fluorescence assay that avoids the problematic PCR and gel analysis of the current "gold-standard" assay.
متن کاملTwo Step Flow Theory
influence of media messages History and Orientation The two-step flow of communication hypothesis was first introduced by Paul Lazarsfeld, Bernard Berelson, and Hazel Gaudet in The People's Choice, a 1944 study focused on the process of decision-making during a Presidential election campaign. These researchers expected to find empirical support for the direct influence of media messages on voti...
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ژورنال
عنوان ژورنال: The Journal of the Society of Chemical Industry, Japan
سال: 1965
ISSN: 0023-2734,2185-0860
DOI: 10.1246/nikkashi1898.68.9_1655