Identifying the Mechanism of Reduced Flavin Transfer in Alkanesulfonate Monooxygenase System
نویسندگان
چکیده
منابع مشابه
Altered mechanism of the alkanesulfonate FMN reductase with the monooxygenase enzyme.
The two-component alkanesulfonate monooxygenase system from Escherichia coli is comprised of an FMN reductase (SsuE) and a monooxygenase enzyme (SsuD) that together catalyze the oxidation of alkanesulfonate to the corresponding aldehyde and sulfite products. To determine the effects of protein interactions on catalysis, the steady-state kinetic parameters for SsuE were determined in single-enzy...
متن کاملDetection of protein-protein interactions in the alkanesulfonate monooxygenase system from Escherichia coli.
The two-component alkanesulfonate monooxygenase system utilizes reduced flavin as a substrate to catalyze a unique desulfonation reaction during times of sulfur starvation. The importance of protein-protein interactions in the mechanism of flavin transfer was analyzed in these studies. The results from affinity chromatography and cross-linking experiments support the formation of a stable compl...
متن کاملBacterial flavin-containing monooxygenase is trimethylamine monooxygenase.
Flavin-containing monooxygenases (FMOs) are one of the most important monooxygenase systems in Eukaryotes and have many important physiological functions. FMOs have also been found in bacteria; however, their physiological function is not known. Here, we report the identification and characterization of trimethylamine (TMA) monooxygenase, termed Tmm, from Methylocella silvestris, using a combin...
متن کاملRegulation of Flavin-Containing Monooxygenase
The flavin-containing monooxygenases (FMOs) are important for the oxidation of a variety of environmental toxicants, natural products, and therapeutics. Consisting of six family members (FMO1–5), these enzymes exhibit distinct but broad and overlapping substrate specificity and are expressed in a highly tissueand species-selective manner. Corresponding to previously identified regulatory domain...
متن کاملDevelopmental Regulation of Flavin - Containing Monooxygenase
approved: David E. Williams The mammalian FMO represents a multigene family which oxygenates a large number of xenobiotics. No physiological role has been determined for FMO, although synthesis of disulfide bonds and detoxification of dietary chemicals have been suggested. Five FMO gene subfamilies, each containing a single gene, have been identified. In this study, we determined the patterns o...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2013
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.27.1_supplement.789.21