Identifying parental chromosomes and genomic rearrangements in animal hybrid complexes of species with small genome size using Genomic In Situ Hybridization (GISH)

Identifying parental chromosomes and genomic rearrangements in animal hybrid complexes of species with small genome size using Genomic In Situ Hybridization (GISH)

Genomic In Situ Hybridization (GISH) is a powerful tool to identify and to quantify genomic constituents in allopolyploids, and is mainly based on hybridization of highly and moderate repetitive sequences. In animals, as opposed to plants, GISH has not been widely used in part because there are technical problems in obtaining informative results. Using the allopolyploid Squalius alburnoides Ste...

Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora.

The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC1) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133). GISH was performed using genomic probes prepared with the DNA from the paternal geni...

The Choromosomal Assesment of Salt Tolerant Substituted Tritipyrum Using Genomic Fluorescentin situ Hybridization (FISH)

the study of chromosome constitution in secondary tritipyrum genotypes (f2) by genomic in situ hybridization technique (gish)

to remove unfavorable traits of new cereal tritipyrum (2n=6x=42, aabbebeb), various crosses between primary tritipyrum lines (female) with iranian bread wheat varieties (2n=6x=42, aabbdd) was performed and a f2 selfing population consisted of 1810 possible secondary tritipyrum seeds (2n=6x=42, aabbd(0-14) eb(0-14)) were produced. the chromosome constitution and homology index of 11 plants of th...

Application of Genomic In Situ Hybridization in Horticultural Science

Molecular cytogenetic techniques, such as in situ hybridization methods, are admirable tools to analyze the genomic structure and function, chromosome constituents, recombination patterns, alien gene introgression, genome evolution, aneuploidy, and polyploidy and also genome constitution visualization and chromosome discrimination from different genomes in allopolyploids of various horticultura...