Identification of Ziziphi Spinosae Semen from Its Adulterants Based on RAPD

Background Ziziphi spinosae semen (ZSS), dry mature seeds of Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H.F. Chow., are widely used in the treatment insomnia, fright palpitations, and profuse dreaming medical treatments. The counterfeit species ZSS, mauritianae (ZMS), Hovenia dulcis Thunb (HDT) from incurva Roxb. acerba Lindl., respectively. However, pharmacopeia is not recorded for ZMS HDT. In this article, we reviewed identification methods ZMS, Materials Methods random amplified polymorphic DNA (RAPD) technique was to identify ZSS its copies. Plant genome extracted Z. Mill., Roxb., H. Lindl. Random primers were designed amplify genome. products detected by agarose gel electrophoresis. Results By analyzing polymorphism product fragment, fingerprint maps successfully constructed.