Identification of the E5 open reading frame of human papillomavirus type 16
نویسندگان
چکیده
منابع مشابه
Membrane orientation of the human papillomavirus type 16 E5 oncoprotein.
The E5 protein of human papillomavirus type 16 is a small, hydrophobic protein that localizes predominantly to membranes of the endoplasmic reticulum (ER). To define the orientation of E5 in these membranes, we employed a differential, detergent permeabilization technique that makes use of the ability of low concentrations of digitonin to selectively permeabilize the plasma membrane and saponin...
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Cervical cancer is a progressive disease with an onset of one to two decades on average. During the productive replication stage, the Human papillomavirus (HPV) genome is maintained episomally in the infected cervical epithelium and early gene products, including E5, are expressed. Therefore, E5 has a potential to contribute to the HPV-associated carcinogenic process. In invasive malignancies, ...
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A human papillomavirus type 11 (HPV-11) early gene promoter, PE1--E4, in the E7 open reading frame and involved in the transcription of E1--E4 and E1--E4, L1 mRNAs was characterized by chloramphenicol acetyl transferase (CAT) assay, gel-shift assay, in vitro transcription, and primer extension. By CAT assay with CAT-expression plasmids containing various portions of the HPV-11 genome from nucle...
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Human papillomavirus (HPV) infection is a prerequisite of developing cervical cancer, approximately half of which are associated with HPV type 16. HPV 16 encodes three oncogenes, E5, E6, and E7, of which E5 is the least studied so far. Its roles in regulating replication and pathogenesis of HPV are not fully understood. Here we utilize high-throughput screening to coordinately investigate the e...
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The E7 open reading frame of human papillomavirus type 16 (HPV-16) encodes a protein that can immortalize primary rat cells, cooperate with the ras oncoprotein to transform low passage rat cells and transform established rodent cells to anchorage independence. The immortalizing and cooperation functions have been investigated using a series of point mutations that introduce single amino acid ch...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1988
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.62.3.1071-1075.1988