Identification of T4D bacteriophage gene product 12 as the baseplate zinc metalloprotein
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Identification of T4D bacteriophage gene product 12 as the baseplate zinc metalloprotein.
The short tail fibers on the baseplate of the Escherichia coli T4D bacteriophage gene 12 have been found to be a zinc metalloprotein. This conclusion is based on several lines of evidence. (a) Analysis of highly purified gene 12 protein showed that it contained 1.0 f 0.1 zinc atom/55,000-dalton monomer. (b) The presence of a zinc-chelating agent, such as o-phenanthroline, labilized the purified...
متن کاملStructure of bacteriophage T4 gene product 11, the interface between the baseplate and short tail fibers.
Bacteriophage T4, like all other viruses, is required to be stable while being transmitted from host to host, but also is poised to eject efficiently and rapidly its double-stranded DNA genome to initiate infection. The latter is coordinated by the recognition of receptors on Escherichia coli cells by the long tail fibers and subsequent irreversible attachment by the short tail fibers. These fi...
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The protein product of gene 11 of bacteriophage T4D was purified to apparent homogeneity from a bacteriophage culture in which this particular structural protein had not yet become incorporated into the mature bacteriophage particle. Monomer and apparent trimer molecular weights were determined to be approximately 24,000 and 70,000, respectively, and an amino acid analysis was performed. The ki...
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Many bacteriophages, such as T4, T7, RB49, and phi29, have complex, sometimes multilayered, tails that facilitate an almost 100% success rate for the viral particles to infect host cells. In bacteriophage T4, there is a baseplate, which is a multiprotein assembly, at the distal end of the contractile tail. The baseplate communicates to the tail that the phage fibers have attached to the host ce...
متن کاملPolynucleotide ligase in bacteriophage T4D recombination.
Following infection of E. coli B with ligase-deficient rII bacteriophage T4D recombination between linked markers is increased 4.2 fold and heterozygote frequency increased 2.3 fold. In such infection recombination occurs at a rapid rate for an extended period. This is in contrast to the time course of recombination observed in wild-type, lysis-inhibited, or lysis-defective (gene t defective) i...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)30409-x