Identification of Putative Parental Species of Yuzu (Citrus junos Sieb. Ex Tanaka) by Comparative Analyses of Variations in Chloroplast Genomes and Nuclear Single-copy Genes of Citrus

Yuzu (Citrus junos Sieb. Ex Tanaka) is one of the most important Citrus species in China, Japan, and Korea. assumed to be a natural hybrid. However, concrete evidence supporting its hybrid origin has not been reported yet. To identify putative maternal species, nucleotide sequences five hypervariable regions covering 17,531 bp citrus chloroplast genomes were obtained from yuzu. A phylogenetic tree based on these showed that yuzu was closely related Ichang papeda (C. cavaleriei), suggesting might seed parent paternal homologs 103 single-copy genes found among identified transcriptome de novo assembled using RNA-seq reads. Eighty homologous transcriptome. sequence variations 80 other mandarin. assess origins genes, single polymorphisms (SNPs) yuzu, papeda, mandarin, citron, pummelo analyzed, SNP genotypes by visual investigation alignments Forty-three out originate hybrids between Taken together, results this study indicate mandarin parental