Identification of population of bacteria from culture negative surgical site infection patients using molecular tool

Abstract Background Managing surgical site infections, with negative culture report in routine diagnosis is a common dilemma microbiology accounting more than 30% worldwide. The present study attempted to identify the presence of bacterial spp. if any wound aspirates/swabs infections hospitalised patients using molecular tools. Methods Ninety-seven post-operative SSI whose swabs/aspirate were conventional aerobic after 72 h incubation analysed by 16S rRNA gene specific broad range PCR. amplified DNA fragments sequenced Sanger sequencing method and homology sequence matched NCBI BLAST (NCBI, USA) Results Of 97 patients, based PCR assay could pathogen 53(54.63%) cases, which 29 isolates supposed be viable but non-culturable bacteria (VBNC), 07 obligatory anaerobes 13 unculturable bacteria, 04 poly infections. Conclusions Our highlights usefulness detecting VBNC, regardless how well may or not grow culture. Measures should taken use anaerobic system along detect VBNC where Gram stain positive for better patient care.