Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library

Identification of the protective and toxic antigens of Corynebacterium pseudotuberculosis.

Cameron (1964) showed that the immunizing property of Corynebacterium pseudotuberculosis cells was closely associated with the toxicity of cell endoplasm and the ability of formalin-killed cells to produce sterile abscesses. He also found that the presence of toxic factors in the cells depended on the culture medium to a large extent. Furthermore Cameron & Swart (1965) developed a fluid medium ...

Isolation of Corynebacterium pseudotuberculosis from Camel in Iran

Rapid identification of novel antigens of Salmonella Enteritidis by microarray-based immunoscreening

We report on an approach to rapidly screen thousands of Salmonella Enteritidis proteins with the goal of identifying novel immunodominant proteins. We used a microarray-based system that warrants high throughput and easy handling. Seven immunogenic candidates were selected after screening. Comparative analyses by ELISA and microarrays manifested their immunodominant character. The large repetit...

Identification and Characterization of a New Autoimmune Protein in Membranous Nephropathy by Immunoscreening of a Renal cDNA Library

Membranous Nephropathy (MN) represents a large amount of Nephrotic Syndromes in the adult population and its definitive diagnosis is currently carried out through biopsy. An autoimmune condition has been demonstrated in idiopathic MN (iMN) in which some kidney structures are targeted by patient autoantibodies. Some candidate antigens have been described and other likely involved target proteins...

Cloning, nucleotide sequence, and expression in Escherichia coli of the phospholipase D gene from Corynebacterium pseudotuberculosis.

The phospholipase D (PLD) gene from Corynebacterium pseudotuberculosis has been cloned, sequenced, and expressed in Escherichia coli. Analysis of DNA sequence data reveals a major open reading frame encoding a 31.4-kilodalton protein, a size consistent with that estimated for the PLD protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Comparison of these data with the amino-te...