Identification of Lactate Dehydrogenase Isoenzymes by Rapid Kinetics
نویسندگان
چکیده
منابع مشابه
Identification of lactate dehydrogenase isoenzymes by rapid kinetics.
Stopped-flow kinetics indicate that human and chicken heart-type(4) lactate dehydrogenases (LDH) become inhibited as DPNH is oxidized in the presence of high concentrations of pyruvate. This inhibition is much less marked with the human and chicken muscle-type(4) enzymes. The initial rates and the difference in inhibition between the two types of enzyme have made it possible to determine the am...
متن کاملTissue distribution of mammalian lactate dehydrogenase isoenzymes.
system of Jervis [7] is used here.) Previous electrophoretic examination of the LDH of most other plants was carried out with extracts from leaves [1,2j. Such a study of potato leaves would not have revealed the existence of isoenzymic forms in this species. We have, therefore, examined extracts from various regions of a number of plants: roots, stem-base, upper stems, leaves and any special ti...
متن کاملUnusual variant of lactate dehydrogenase isoenzymes.
Thirteen electrophoretic bands of lactate dehydrogenase isoenzyme activity were detected in the serum of a 69-year-oldNegro woman who died of pseudomonas pneumonia. No evidence of tumor was found at necropsy, and additional studies of the enzymatic activity of crude extracts of liver, lung, spleen, kidney, brain, skeletal muscle, lymph node, and heart revealed 1, 2, 3, 4, and 5 separate lsoenzy...
متن کاملClinical applications of lactate dehydrogenase isoenzymes.
A quantitative electrophoretic test for the determination of lactate dehydrogenase isoenzymes was applied to the analysis of human tissues, cells and fluids in order to obtain their normal isoenzyme patterns and to form a reference record. The same test was employed in the analysis of serum samples from patients with defined pathological conditions. The abnormal serum isoenzyme patterns were co...
متن کاملLactate dehydrogenase isoenzymes of human semen.
1. A lactate dehydrogenase isoenzyme present in human spermatozoa and semen was isolated and characterized biochemically in term of its pH for optimum activity and by means of K(m) values for lactate, NAD(+) and NAD analogues. The results were compared with those obtained with the human heart-type and the liver-type lactate dehydrogenase isoenzymes. 2. The enzyme was characterized by its resist...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1972
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.69.7.1761