Identification of immunodominant epitope of F1 antigen ofYersinia pestis
نویسندگان
چکیده
منابع مشابه
molecualr cloning of the capsular antigen f1 of yersinia pestis in pbad/giii plasmid
yersinia pestis which is the causative agent of pneumonic plague and distributed in all continents has led to many deaths during the history. because of its high mortality rate, it must be diagnosed and treated at the earliest time post infection and therefore, rapid diagnostic tests are required. in the present study, we cloned the coding sequence of f1 capsular antigen of the bacteria in the ...
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Targeting antigens to dendritic cells (DCs) by using hybrid monoclonal antibodies (mAbs) directed against DC receptors is known to improve activation and support long-lasting T cell responses. In the present work, we used the mAb αDEC205 fused to the Trypanosoma cruzi amastigote surface protein 2 (ASP-2) to identify a region of this protein recognized by specific T cells. The hybrid αDEC-ASP2 m...
متن کاملMolecualr Cloning of the capsular antigen F1 of Yersinia pestis in pBAD/gIII plasmid
Yersinia pestis which is the causative agent of pneumonic plague and distributed in all continents has led to many deaths during the history. Because of its high mortality rate, it must be diagnosed and treated at the earliest time post infection and therefore, rapid diagnostic tests are required. In the present study, we cloned the coding sequence of F1 capsular antigen of the bacteria in the ...
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Yersinia pestis, the causative organism of plague, produces a capsular protein (fraction 1 or F1 antigen) that is one of the major virulence factors of the bacterium. We report here the production, structural and immunological characterisation of a recombinant F1 antigen (rF1). The rF1 was purified by ammonium sulfate fractionation followed by FPLC Superose gel filtration chromatography. Using ...
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Yersinia pestis F1 antigen-loaded poly(DL-lactide-co-glycolide)/polyethylene glycol (PEG) (PLGA/PEG) microspheres were produced using a water-in-oil-in-water emulsion/solvent extraction technique and assayed for their percent yield, entrapment efficiency, surface morphology, particle size, zeta potential, in vitro release properties, and in vivo animal protect efficacy. The Y. pestis F1 antigen...
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ژورنال
عنوان ژورنال: FEMS Immunology & Medical Microbiology
سال: 2000
ISSN: 0928-8244,1574-695X
DOI: 10.1111/j.1574-695x.2000.tb01426.x