Identification of Gene Encoding Organophosphorus Hydrolase (OPH) Enzyme in Potent Organophosphates-degrading Bacterial Isolates
نویسندگان
چکیده
منابع مشابه
a review on engineering of organophosphorus hydrolase (oph) enzyme
organophosphorus chemicals are compounds which have been used as pesticides and insecticides in agriculture. they’re also used as nervous agents and have raised many problems for human and environment. among the most important methods of decontamination from these compounds are biodegradation methods. using oph enzyme in degradation the mentioned compounds is seen as one of the desirable ways, ...
متن کاملAminoalcohol-Induced Activation of Organophosphorus Hydrolase (OPH) towards Diisopropylfluorophosphate (DFP)
Aminoalcohols have been addressed as activating buffers for alkaline phosphatase. However, there is no record on the buffer activation regarding organophosphorus hydrolase (OPH). Here we reported the activating effects of aminoalcohols on OPH-catalyzed hydrolysis of diisopropylfluorophosphate (DFP), an analog molecule of G-type warfare agents. The kinetic parametors kcat, Vmax and kcat/Km in th...
متن کاملEvaluation of the organophosphorus hydrolase enzyme activity in creams and investigation of its stability
The main purpose of this project is investigation of the organophosphorus hydrolase (OPH) enzyme activity in water in oil (w/o) and oil in water (o/w) creams and investigation of the OPH enzyme stability in formulated creams. OPH enzyme was extracted and purified from strain flavobacterium. The w/o and o/w creams were prepared using different formulations. In order to achieve an emulsion with m...
متن کاملexpression and characterization of bacterial organophosphorus hydrolase in pichia pastoris with the intent to degrade organophosphate neurotoxins
objective: organophosphorus hydrolase (oph) is a homodimeric enzyme that can hydrolyze phosphoester bonds and reduce the toxicity of organophosphorus compounds. this makes oph a suitable element for the biodegradation of these compounds. methods: we successfully cloned the oph gene from pseudomonas diminuta, after optimization for pichia pastoris, into a yeast expression vector (ppiczαb). afte...
متن کاملOrganophosphate compounds and their biodegradation; using enzymes as an increased efficiency approach
Organophosphorus compounds are widely used in pesticides, insecticides in agriculture and as nervous chemical agents. These chemicals inhibit the acetylcholinesterase enzyme activity that is responsible for the nervous impulse in organisms. This effect leads to an increase in acetylcholine level and finally neuronal complications. Many methods are used to degrade and decontaminate these compou...
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ژورنال
عنوان ژورنال: Journal of Environmental Science and Technology
سال: 2018
ISSN: 1994-7887
DOI: 10.3923/jest.2018.175.189