Identification of effective primers for varieties of winter rye Secale cereals L.

Rye (Secale cereale L.) is important food crop in Russia. The study of the genetic diversity crops carried out using molecular markers. To identify effective primers, ISSR (Inter Simple Sequence Repeats), a method for determining DNA polymorphism, was used.The selection primers according to largest number polymorphic fragments, their clear amplification and reproducibility repeated PCR. Of 22 analyzed with total S. cereale, 5 demonstrated high efficiency studied winter rye varieties. For further analysis following four dinucleotide (M1 (AC)8CG, ISSR-5 (AG)8CA, CR-215 (CT)6TG, CR-218 (GA)6CC) one trinucleotide primer X10 (AGC)6C. identified will make it possible determine