Identification of critical pathways and potential therapeutic targets in poorly differentiated duodenal papilla adenocarcinoma

Abstract Background Duodenal papilla carcinoma (DPC) is a rare malignancy of the gastrointestinal tract with high recurrence rate, and pathogenesis this highly malignant neoplasm yet to be fully elucidated. This study aims identify key genes further understand biology underlying molecular alterations driving DPC, which could potential diagnostic or therapeutic targets. Methods Tumor samples three DPC patients were collected integrating RNA-seq analysis tumor tissues matched normal performed discover differentially expressed (DEGs). Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment carried out bio-functions Protein–protein interaction (PPI) network was constructed for functional modules identification hub genes. qRT-PCR clinical conducted validate expression level Results A total 110 DEGs identified from our data, GO KEGG analyses showed that mainly enriched in multiple cancer-related functions pathways, such as cell proliferation, IL-17signaling pathway, Jak-STAT signaling PPAR pathway. The PPI screened five including IL-6, LCN2, FABP4, LEP MMP1, core value validated by qRT-PCR. work suggested targets DPC. Discussion current may provide new insight into exploration serve indicator novel target.