Identification of Circular RNA hsa-circ-0006969 as a Novel Biomarker for Breast Cancer

Background: To investigate the characteristics of circular RNA hsa-circ-0006969 in breast cancer and identify it as a novel biomarker for cancer. Methods: Three (BC) patient tissues were selected to perform human circRNA microarray analysis. GeneSpring 13.0 (Agilent) software was applied analyzing data. Another 116 BC patients recruited verification. Hsa-circ-0006969 found potential diagnostic biomarker. The structure predicted by circPrimer1.2 software. MiRanda v3.3, hybrid 2.1, Cytoscape 3.6.0 used predicting networks circRNA-miRNA. T-test, Curve regression, ROC analysis certify values hsa-circ-0006969. Using SPSS25.0 Statistical Results: 546 higher expression 1475 lower circRNAs identified. Four filtrated further significantly low expressed peripheral blood. confirmed correlation with (AUC = 0.965) blood 0.842), Grade 1 0.639), ER-positive 0.612), TNM I 0.693), II 0.712), III 0.757), early stage(I, II) 0.709). presented more effective tumor metastasis 0.784) compared CA153 0.752). Conclusion: could be diagnosis treatment BC.