Identification of Adducts Formed by Reaction of N-Acetoxy-3,5-dimethylaniline with DNA
نویسندگان
چکیده
منابع مشابه
Mitosene-DNA adducts. Characterization of two major DNA monoadducts formed by 1,10-bis(acetoxy)-7-methoxymitosene upon reductive activation.
Reductive activation of racemic 1,10-bis(acetoxy)-7-methoxymitosene WV15 in the presence of DNA, followed by enzymatic digestion and HPLC analysis, revealed the formation of various DNA adducts. Reduction is a necessary event for adduct formation to occur. This reductive activation was performed under hypoxic conditions in various ways: (1) chemically, using a 2-fold excess of sodium dithionite...
متن کاملIdentification of the major adducts formed by reaction of benzo(a)pyrene diol epoxide with DNA in vitro.
Covalent binding of the benzo[a]pyrene metabolite (+/-)7beta,8alpha-dihydroxy-9alpha,10alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene to calf thymus DNA was investigated. Enzymatic hydrolysis of the carcinogen-modified DNA and subsequent separation via reversed-phase high-pressure liquid chromatography resulted in the detection and isolation of seven distinct products. High-resolution mass spect...
متن کاملTamoxifen metabolic activation: comparison of DNA adducts formed by microsomal and chemical activation of tamoxifen and 4-hydroxytamoxifen with DNA adducts formed in vivo.
One of our laboratories recently showed by 32P-postlabeling that administration of tamoxifen to mice induces two groups of hepatic DNA adducts comprising two major spots, nos. 3 and 5, respectively. 4-Hydroxytamoxifen and alpha-hydroxytamoxifen appear to be the proximate metabolites of groups I and II adducts, respectively. The relative significance of these two adduct groups for tamoxifen carc...
متن کاملContribution of monofunctional adducts formed by furocoumarins with DNA to the inhibition of nucleic acids synthesis.
In addition to the bifunctional adducts (cross-linkages), that furocoumarins on radiation at 365 nm form in DNA, monofunctional adducts also proved able to inhibit the nucleic acid synthesis in Ehrlich ascites tumor cells.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemical Research in Toxicology
سال: 2007
ISSN: 0893-228X,1520-5010
DOI: 10.1021/tx700306c