Identification of a novel transcription factor under long-term drought resistance in highland barley: a DNA affinity purification sequencing-based transcriptomic analysis

Abstract Stress emanating from drought condition is one of the inevitable conditions that occurs in many environments and spread across borders results severe hindrance to morphology, physiology, biochemistry molecular attributes plants. Highland barley tolerance has been demonstrated be a polygenic related characteristic genetic composition can assist dissecting gene network(s) controlling needs adequately understood. DNA affinity purification sequencing (DAP-seq) shown contribute ways making pure some protein with rare sequence-specific binding help understand dynamics under varying exposure time drought. The present study aimed at identifying novel transcription factors (TFs) highland are key resistance through DAP-seq-based transcriptomic analysis. experimental design included two hulless accessions; drought-resistant Sheera 10 (X) drought-sensitive 5171–7 (W), were both subjected short-term (4 h) long-term (48 (T1 T5, respectively), control group (CK) involving not subjecting accessions any treatment. Through transcriptome analysis, candidate factor (GATA family; (bHLH, MYB-related, GARP-G2-like, bZIP, HB-HD-ZIP, C2H2, SET, mTERF, AP2/ERF-ERF, ARID, NAC, GARP-ARR-B, C2C2-GATA, FAR1, Trihelix, NF-YB, B3 AUX/IAA) was found. motif obtained found consistent GATA factor. DAP-seq highlighted differential expression target which verified by RT-qPCR. associated genes cluster structural IP_HOR_1, IP_HOR_2 In_HOR locus. RT-qPCR, it also proved expressions indeed upregulated. TF HOVUSG2784400 confirmed responsible stress exposure, regulates genes, thereby improving barley. Graphical