Identification and screening of host proteins interacting with ORFV-ORF047 protein
نویسندگان
چکیده
Abstract Background Orf virus (ORFV) is a member of the genus Parapoxvirus and family Poxviridae. The has worldwide distribution infects sheep, goats, humans, wild animals. However, due to complex structure poxvirus, underlying mechanism entry infection by ORFV remains largely unknown. ORF047 encodes protein named L1R. Poxviral L1R serves as receptor-binding blocks binding independently glycosaminoglycans (GAGs). study aimed identify host interaction partners ORF047. Methods Yeast two-hybrid cDNA library sheep testicular cells was applied screen targets with bait. cloned into pBT3-N vector expressed in NMY51 yeast strain. Then, expression bait proteins validated Western blot analysis. Results S heep SERP1and PABPC4 were identified target ORF047, Co-IP assay further verified their interaction. Conclusions New cell found interact might involve viral mRNA translation replication.
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ژورنال
عنوان ژورنال: Virology Journal
سال: 2021
ISSN: ['1743-422X']
DOI: https://doi.org/10.1186/s12985-021-01499-y