Identification and molecular characterization of Carnation mottle virus Brazilian isolates from carnation
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence and genome organization of carnation mottle virus RNA.
The complete nucleotide sequence of carnation mottle genomic RNA (4003 nucleotides) is presented. The sequence was determined for cloned cDNA copies of viral RNA containing over 99% of the sequence and was completed by direct sequence analysis of RNA and cDNA transcripts. The sequence contains two long open reading frames which together can account for observed translation products. One transla...
متن کاملCharacterization of an Indian isolate of Carnation mottle virus infecting carnations
CURRENT SCIENCE, VOL. 88, NO. 4, 25 FEBRUARY 2005 594 The nucleotide sequence data here have been submitted in the EMBL nucleotide database under the accession numbers AJ549954 (CP), AJ584842 (P7) and AJ584843 (P9). Sequences of designed primers AOSCarMVCPu and AOSCarMVCPd have been submitted to the EMBL database under accession number AJ566191 and AJ566192 respectively. *For correspondence. (e...
متن کاملStructural properties of carnation mottle virus p7 movement protein and its RNA-binding domain.
Plant viral movement proteins (MPs) participate actively in the intra- and intercellular movement of RNA plant viruses to such an extent that MP dysfunction impairs viral infection. However, the molecular mechanism(s) of their interaction with cognate nucleic acids are not well understood, partly due to the lack of structural information. In this work, a protein dissection approach was used to ...
متن کاملUncoating Mechanism of Carnation Mottle Virus Revealed by Cryo-EM Single Particle Analysis
Genome uncoating is a prerequisite for the successful infection of plant viruses in host plants. Thus far, little is known about the genome uncoating of the Carnation mottle virus (CarMV). Here, we obtained two reconstructions of CarMV at pH7 in the presence (Ca-pH7) and absence (EDTA-pH7) of calcium ions by Cryo-EM single particle analysis, which achieved 6.4 Å and 8 Å resolutions respectively...
متن کاملAn encapsidated, subgenomic messenger RNA encodes the coat protein of carnation mottle virus.
The translation strategy of carnation mottle virus (CarMV) in vitro has been generally assumed to involve internal initiation events on full-length, genomic RNA (4.3 kb). We suggest that this is, at least in part, incorrect. Encapsidated RNA, fractionated on denaturing sucrose gradients, or total RNA from CarMV-infected leaves, fractionated under non-denaturing conditions, was translated in an ...
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ژورنال
عنوان ژورنال: Horticultura Brasileira
سال: 2015
ISSN: 0102-0536
DOI: 10.1590/s0102-053620150000200019