Identification and immunochemical characterization of a germ tube specific antigen of Candida albicans
نویسندگان
چکیده
منابع مشابه
New monoclonal antibody specific for Candida albicans germ tube.
Hydrophobic components of the germ tube of the dimorphic pathogenic fungus Candida albicans were used as immunogens to prepare monoclonal antibodies (MAbs). Among the resulting MAbs, one (MAb 16B1-F10) was shown by indirect immunofluorescence to be specific to the surface of the mycelium phase of the C. albicans and C. stellatoidea species. No labeling of any other genera and Candida species te...
متن کاملSerum tube identification of Candida albicans.
The production of filaments (germ tubes) by cells of Candida albicans in serum tubes permits presumptive identification to be made within two to three hours. The proportion of yeast-like cells forming filaments is progressively decreased with increasing cell concentration. The test is effective over a comparatively wide range of temperatures and using different types and concentrations of sera.
متن کاملIdentification of the dialysable serum inducer of germ-tube formation in Candida albicans.
Yeast cells of Candida albicans are induced by serum at 37 degrees C to produce germ tubes, the first step in a transition from yeast to hyphal growth. Previously, it has been shown that the active component is not serum albumin but is present in the dialysable fraction of serum. In this study, serum induction of germ-tube formation is shown to occur even in the presence of added exogenous nitr...
متن کاملHemin induces germ tube formation in Candida albicans.
Hemin induced germination of Candida albicans blastoconidia when cells grown up to the early exponential phase were shifted from 28 to 37 degrees C (70 to 75% of cells exhibited germ tubes). N-Acetyl-D-glucosamine (GlcNAc), another inducer of myceliation in this fungus, caused a similar effect. The combination of hemin and GlcNAc resulted in a higher percentage (95%) of blastoconidial germinati...
متن کاملIdentification and characterization of a Candida albicans mating pheromone.
Candida albicans, the most prevalent fungal pathogen of humans, has recently been shown to undergo mating. Here we describe a mating pheromone produced by C. albicans alpha cells and show that the gene which encodes it (MFalpha) is required for alpha cells, but not a cells, to mate. We also identify the receptor for this mating pheromone as the product of the STE2 gene and show that this gene i...
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ژورنال
عنوان ژورنال: FEMS Immunology and Medical Microbiology
سال: 1993
ISSN: 0928-8244
DOI: 10.1016/0928-8244(93)90068-f