Identification and Expression of a Rat Fatty Acid Elongase Involved in the Biosynthesis of C18 Fatty Acids

Identification and expression of a rat fatty acid elongase involved in the biosynthesis of C18 fatty acids.

A major part of the palmitic acid (C16:0) generated by fatty acid synthase is converted into stearic acid (C18:0) via carbon chain elongation. Here, we describe the cloning and expression of a rat hepatic enzyme, rELO2, responsible for the elongation of C16:0, presumably at the condensing reaction. Heterologous expression experiments in a yeast, Saccharomyces cerevisiae, demonstrated the elonga...

Transformation of Rapeseed (Brassica napus L.) Plants with Sense and Antisense Constructs of the Fatty Acid Elongase Gene

The biosynthetic pathways of saturated and unsaturated fatty acids consist of many steps controlled by various enzymes. One of the methods for improving oil quality is to change the fatty acid profile through genetic manipulation which requires isolation and characterization of the genes and other cis-acting elements, such as the promoter, involved in fatty acid biosynthesis. b-ketoacyl-CoA syn...

Fatty Acid Profles of Ram's Sperm after Removing Some Fatty Acid Sources from the Diets and Persistency of Fatty Acids in Sperm

MOLECULAR ANALYSIS OF FATTY ACID PEROXYGENASE INVOLVED IN THE BIOSYNTHESIS OF EPOXY FATTY ACIDS IN OATS (Avena sativa)

The biosynthesis of fatty acids in rat liver.

During the past several years, remarkable progress has been made in elucidating biological fatty acid oxidation, and it is now clearly established that the oxidation of acyl CoA’ derivatives proceeds in the mitochondria (1) in a stepwise manner (2, 3) by a series of reactions closely resembling the hypothetical route proposed by Knoop. However, the biosynthesis of fatty acids has been less well...