Hypoxia preconditioned bone marrow-derived mesenchymal stromal/stem cells enhance myoblast fusion and skeletal muscle regeneration
نویسندگان
چکیده
Abstract Background The skeletal muscle reconstruction occurs thanks to unipotent stem cells, i.e., satellite cells. cells remain quiescent and localized between myofiber sarcolemma basal lamina. They are activated in response injury, proliferate, differentiate into myoblasts, recreate myofibers. progenitor support regeneration, which could be disturbed by extensive damage, sarcopenia, cachexia, or genetic diseases like dystrophy. Many lines of evidence showed that the level oxygen regulates course cell proliferation differentiation. Methods In present study, we analyzed hypoxia impact on human pig bone marrow-derived mesenchymal stromal (MSC) mouse myoblast proliferation, differentiation, fusion. Moreover, influence transplantation MSCs cultured under hypoxic conditions regeneration was studied. Results We increased VEGF expression improved myogenesis vitro. Transplantation preconditioned injured muscles resulted engraftment formation new vessels. Conclusions suggested SDF-1 secreted played an essential role angiogenesis. Importantly, more efficiently engrafted muscles; however, they did not undergo myogenic
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ژورنال
عنوان ژورنال: Stem Cell Research & Therapy
سال: 2021
ISSN: ['1757-6512']
DOI: https://doi.org/10.1186/s13287-021-02530-3