Hypo‐glycosylated Human Follicle–Stimulating Hormone (hFSH 21 ) Exhibits Higher Endocytic Rate for Recombinant hFSH Receptor than Fully‐glycosylated hFSH (hFSH 24 )

Recombinant human follicle-stimulating hormone (r-hFSH) plus recombinant luteinizing hormone versus r-hFSH alone for ovarian stimulation during assisted reproductive technology: systematic review and meta-analysis

BACKGROUND The potential benefit of adding recombinant human luteinizing hormone (r-hLH) to recombinant human follicle-stimulating hormone (r-hFSH) during ovarian stimulation is a subject of debate, although there is evidence that it may benefit certain subpopulations, e.g. poor responders. METHODS A systematic review and a meta-analysis were performed. Three databases (MEDLINE, Embase and CE...

Hypo-glycosylated human follicle-stimulating hormone (hFSH21/18) is much more active in vitro than fully-glycosylated hFSH (hFSH24)

Hypo-glycosylated hFSH(21/18) (possesses FSHβ(21) and FSHβ(18)bands) was isolated from hLH preparations by immunoaffinity chromatography followed by gel filtration. Fully-glycosylated hFSH(24) was prepared by combining the fully-glycosylated FSHβ(24) variant with hCGα and isolating the heterodimer. The hFSH(21/18) glycoform preparation was significantly smaller than the hFSH(24) preparation and...

Draft guideline on non-clinical and clinical development of similar biological medicinal products containing recombinant human follicle stimulating hormone (r-hFSH)

Production rates and metabolic clearance rates of human follicle-stimulating hormone in premenopausal and postmenopausal women.

The production rates (PR) and the metabolic clearance rates (MCR) of human follicle-stimulating hormone (HFSH) were determined in six pre- and five postmenopausal women. Human FSH (PER-780) labeled with (131)I to specific activities of 50-150 muc/mug was used as a tracer. Both double antibody and trichloroacetic acid (TCA) precipitation techniques were used to determine HFSH-(131)I levels in in...

Solubilization and some characteristics of the follitropin receptor from calf testis.

Immature calf testes were found to be an unusually rich source of follitropin (FSH) receptors. Particulate fractions derived from such testes bound 32% of added biologically active radiolabeled human FSH (12”I-labeled hFSH) and had a binding capacity of 52 x lo-l4 mol/mg of protein. These values are considerably higher than those previously reported for FSH receptors in other types of beef or m...