Hydroxamate formation by anthranilate synthetase of Escherichia coli K12
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Hydroxamate formation by anthranilate synthetase of Escherichia coli K12.
HO' H /H2 0 \ I dOOH chorismic acid anthranilic acid (Gibson and Gibson, 1964; Somerville, unpublished results). In Escherichia coli the catalytically active species is a protein complex formed by aggregation of the products of the E and D genes of the tryptophan operon (Ito and Yanofsky, 1966). Anthranilate synthetase is one of a class of amidotransferase enzymes where L-glutamine serves as th...
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Anthranilate synthetase was purified by ammonium sulfate precipitation and gel filtration from extracts of an episomebearing Escherichia coli mutant grown under conditions of tryptophan pathway derepression. This purification represented an l&fold increase in specific activity over the activity of the derepressedmutant extract. Starch gel electrophoresis and ultracentrifugation revealed only sl...
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ژورنال
عنوان ژورنال: Biochemical and Biophysical Research Communications
سال: 1967
ISSN: 0006-291X
DOI: 10.1016/0006-291x(67)90331-2