Hydrophobic interaction chromatography for the characterization of monoclonal antibodies and related products
نویسندگان
چکیده
منابع مشابه
An alternative assay to hydrophobic interaction chromatography for high-throughput characterization of monoclonal antibodies
The effectiveness of therapeutic monoclonal antibodies (mAbs) is governed not only by their bioactivity, but also by their biophysical properties. Assays for rapidly evaluating the biophysical properties of mAbs are valuable for identifying those most likely to exhibit superior properties such as high solubility, low viscosity and slow serum clearance. Analytical hydrophobic interaction chromat...
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■ ■ Reduced temperature dependence for decreasd on-column degradation IN T RO DU C T IO N While hydrophilic interaction chromatography (HILIC) has been widely used for separating small polar compounds, its application to large biomolecules, other than released glycans, has been surprisingly limited. In large part, this can be attributed to a paucity of suitable HILIC column technology and robus...
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Hydrophobic interaction chromatography (HIC) is commonly used as a polishing step in monoclonal antibody purification processes. HIC offers an orthogonal selectivity to ion exchange chromatography and can be an effective step for aggregate clearance and host cell protein reduction. HIC, however, suffers from the limitation of use of high concentrations of kosmotropic salts to achieve the desire...
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Monoclonal antibodies have proven their potential as therapeutic molecules with applications as diverse as diagnosis, oncology and treatment of autoimmune diseases; the need for their supply is higher than it ever was, with the global market for monoclonal antibodies being expected to grow to nearly $58 billion in 2016. The main challenge for this growth remains the fact these products need to ...
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abstract while task-based instruction is considered as the most effective way to learn a language in the related literature, it is oversimplified on various grounds. different variables may affect how students are engaged with not only the language but also with the task itself. the present study was conducted to investigate language and task related engagement on the basis of the task typolog...
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ژورنال
عنوان ژورنال: Journal of Pharmaceutical and Biomedical Analysis
سال: 2016
ISSN: 0731-7085
DOI: 10.1016/j.jpba.2016.04.004