Hydrogen production in nitrogenase mutants in Anabaena variabilis
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چکیده
منابع مشابه
RNA processing of nitrogenase transcripts in the cyanobacterium Anabaena variabilis.
Little is known about the regulation of nitrogenase genes in cyanobacteria. Transcription of the nifH1 and vnfH genes, encoding dinitrogenase reductases for the heterocyst-specific Mo-nitrogenase and the alternative V-nitrogenase, respectively, was studied by using a lacZ reporter. Despite evidence for a transcription start site just upstream of nifH1 and vnfH, promoter fragments that included ...
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Anabaena variabilis ATCC 29413 has two distinct nitrogenases that function in heterocysts, a conventional Mo-nitrogenase and an alternative V-nitrogenase. Synthesis of these two enzymes was repressed in cells growing with a source of fixed nitrogen, such as ammonium; however, the V-nitrogenase was also repressed by Mo. Expression of the V-nitrogenase which was not affected by V and expression o...
متن کاملCharacterization of genes for a second Mo-dependent nitrogenase in the cyanobacterium Anabaena variabilis.
Anabaena variabilis ATCC 29413 is a filamentous heterocystous cyanobacterium that fixes nitrogen under a variety of environmental conditions. Under aerobic growth conditions, nitrogen fixation depends upon differentiation of heterocysts and expression of either a Mo-dependent nitrogenase or a V-dependent nitrogenase in those specialized cells. Under anaerobic conditions, a second Mo-dependent n...
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Anabaena variabilis fixes nitrogen under aerobic growth conditions in differentiated cells called heterocysts using either a Mo nitrogenase or a V nitrogenase. The nifH1 gene, which encodes the dinitrogenase reductase of the Mo nitrogenase that is expressed only in heterocysts, is cotranscribed with nifD1 and nifK1, which together encode the Mo dinitrogenase. These genes were expressed in the p...
متن کاملThe Expression of a Third Nitrogenase in the Cyanobacterium Anabaena variabilis
Physiological experiments indicate that Anabaena variabilis can express either a Vor a Fe-nitrogenase in addition to the conventional, Mo-containing enzyme complex. The occur rence o f the Fe-nitrogenase in A. variabilis can also be concluded from D N A -D N A hybridiza tion experiments using cloned anfW or nifW probes coding for the smaller subunit (= nitroge nase reductase) o f the Fe-nitr...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2010
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2009.01883.x