Hybrid Flow Bioreactor with All Catalysts Immobilized for Enzymatic Electrosynthesis

Abstract The electrochemical regeneration of the reduced form nicotinamide adenine dinucleotide cofactor (NADH) was realized in a hybrid flow reactor coupling fuel cell technology and redox device, paying attention to robust immobilization all catalysts. rhodium catalyst Rh(Cp✶)(bpy)Cl + covalently immobilized on multi‐walled carbon nanotube (MWCNT) layer association with gas diffusion electrode carefully optimized. High stability activity system were assessed by cyclic voltammetry amperometry reactor. Afterwards, optimal applied NADH‐dependent biosynthesis using lactate dehydrogenase for conversion pyruvate presence concentration as low 10 μM. 79 % Faradaic efficiency achieved remarkable total turnover number (TTN) reached: 2500, 18000, 180000, NADH, Rh complex L‐lactate (LDH), respectively.