Human Hypoxanthine Phosphoribosyltransferase
نویسندگان
چکیده
منابع مشابه
Human hypoxanthine-guanine phosphoribosyltransferase.
A mutant form of human hypoxanthine-guanine phosphoribosyltransferase (HPRTToronto) was isolated from erythrocytes of a male patient with gout due to a partial deficiency of enzyme activity. The tryptic peptides of HPRTToronto were mapped by reverse-phase high pressure liquid chromatography in an attempt to define the precise abnormality in its primary structure. Sequence analysis of the single...
متن کاملHuman Hypoxanthine Phosphoribosyltransferase
Hypoxanthine phosphoribosyltransferase (inosine monophosphate: pyrophosphate phosphoribosyltransferase EC 2.4.2.8) has been purified SO-fold from an acid-treated lysate of human erythrocytes. The average particle weight of the enzyme was estimated by gel filtration at 60,000. Between the pH values of 7.1 and 9.1 no marked difference in the initial velocity of IMP synthesis was observed. Kinetic...
متن کاملHypoxanthine-Guanine Phosphoribosyltransferase Variant
A B S T R A C T We have previously described a 14-yrold boy with hyperuricemia, renal failure, and accelerated purine production resistant in vivo and in vitro to purine analogs. This patient demonstrated normal red cell hypoxanthine-guanine phosphoribosyltransferase (HPRT) heat stability, electrophoresis at high pH, and activity at standard substrate levels. In the present report an abnormal H...
متن کاملChinese Hamster Hypoxanthine-Guanine Phosphoribosyltransferase
Hypoxanthine-guanine phosphoribosyltransferase from Chinese hamster brain, liver, and V79 tissue culture cells appears to have identical structural and catalytic properties. The enzyme has been purified 540-fold to apparent homogeneity from Chinese hamster brain. The native molecular weight is 78,000 to 85,000 determined by Sephadex G-100 column chromatography and acrylamide gel electrophoresis...
متن کاملHypoxanthine-guanine phosphoribosyltransferase-independent toxicity of azathioprine in human lymphoblasts.
1. E. K. McLean, Pharmac. Rev. 22, 429 (1970). 2. B. Meyrick, W. Gamble and L. Reid, Am. J. Physiol. 239, H692 (1980). 3. K. S. Hilliker, T. G. Bell and R. A. Roth, Am. J. Physiol. 242, H573 (1982). 4. J. M. Kay, P. M. Keane, K. L. Suyama and D. Gauthier, Thorax 37, 88 (1982). 5. L. Merkow and J. Kleinerman, Lab. Invest. 15, 547 (1966). 6. C. A. Wagenvoordt and N. Wagenvoordt, Circulation 42, 1...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91838-7