منابع مشابه
FAD-Dependent NAD(P)H Diaphorase
A newly discovered human diaphorase, designated diaphorase-4, which accounts for a major part of the diaphorase activity of most tissues but does not occur in erythrocytes, is described. In contrast with other human diaphorases, it is dependent on FAD for activity after electrophoresis, inhibited by low concentrations of dicoumarol and shows a marked affinity for Cibacron Blue. The molecular we...
متن کامل[Distribution of NADPH diaphorase-positive nerves in human penile tissue].
Recently, nitric oxide (NO) has been thought to be a neuronal messenger to evoke penile erection. NO synthase (NOS)-containing nerve fibers were identified and localized in human penile tissue, but detail distribution of NOS-containing nerve fibers in the human penis was unclear. In the present study we examined their distribution using histochemical staining of NADPH diaphorase (ND), which is ...
متن کاملNADPH-diaphorase in the cat carotid body.
INTRODUCTION AND METHODS. Nitric oxide (NO) has recently emerged as a major modulator of carotid body (CB) chemoreception. This is evidenced by functional and morphological studies. Chemosensory activity is inhibited by NO agonists and disinhibited by antagonists in both in situ and in vitro preparations (1, 2). At the light microscopic level, the NADPH-diaphorase (NADPH-d)/nitric oxide synthas...
متن کاملNeuronal NADPH diaphorase is a nitric oxide synthase.
NADPH diaphorase histochemistry selectively labels a number of discrete populations of neurons throughout the nervous system. This simple and robust technique has been used in a great many experimental and neuropathological studies; however, the function of this enzyme has remained a matter of speculation. We, therefore, undertook to characterize this enzyme biochemically. With biochemical and ...
متن کاملRoles of Triton X-100 in NADPH-diaphorase histochemistry.
Triton X-100 is widely but not universally used in NADPH-diaphorase histochemical staining. We investigated its effect on the staining and examined nitroblue diformazan (NBF) production under the influence of Triton X-100. Exposure of opossum esophagus, intestine, and colon tissues to Triton X-100 before staining enhanced staining of nerve cells and fibers and suppressed staining of non-neural ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1980
ISSN: 0264-6021
DOI: 10.1042/bj1870429