Human Erythrocytes and Resealed Ghosts
نویسندگان
چکیده
منابع مشابه
Organic-acid transport in resealed haemoglobin-containing human erythrocyte 'ghosts'.
The transport of organic acids across the membrane of resealed haemoglobin-containing erythrocyte 'ghosts' prepared by a dialysis technique has been studied. The present work forms part of studies directed towards the use of erythrocyte cellular carriers in enzyme-replacement therapy of inherited metabolic diseases. Oxalic acid, glycollic acid and glyoxylic acid were taken as representative of ...
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Erythrocytes are the most abundant cells in the human body (~5.4 million cells/mm blood in a healthy male and ~ 4.8 million cells/mm blood in a healthy female) having potential carrier capabilities for the delivery of drugs and drug loaded microspheres. Drug-loaded carrier erythrocytes are prepared simply by collecting blood samples from the organism of interest, separating erythrocytes from pl...
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Amongst various carriers explored for target oriented drug delivery, vesicular, microparticulate and cellular carriers meet several criteria rendering them useful in clinical applications. Lymphocytes, leukocytes, platelets, granulocytes and erythrocytes have been proposed as a cellular carrier. Erythrocytes, also known as red blood cells, and have been the most extensively investigated and fou...
متن کاملPreparation and characterization of hormone-sensitive, resealed erythrocyte ghosts.
A method for preparing resealed turkey erythrocyte ghosts is described which utilizes hypotonic lysis and resealing following restoration of isotonicity. The resealed ghosts are isolated above 55% sucrose. The resealed ghosts are shown to be capable of maintaining high intracellular K+ concentrations in the presence of a low K+ extracellular environment. When ATP and an ATP-regenerating system ...
متن کامل[3H]Ouabain binding and Na+, K+-ATPase in resealed human red cell ghosts
The interaction of the cardiac glycoside [3H]ouabain with the Na+, K+ pump of resealed human erythrocyte ghosts was investigated. Binding of [3H]ouabain to high intracellular Na+ ghosts was studied in high extracellular Na+ media, a condition determined to produce maximal ouabain binding rates. Simultaneous examination of both the number of ouabain molecules bound per ghost and the correspondin...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)42420-4