Human erythrocyte phosphofructokinase: Its Purification and some properties
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چکیده
منابع مشابه
Purification and Some Properties of Rabbit Skeletal Muscle Phosphofructokinase.
In spite of an abundant literature (1-15) indicating that phosphofructokinasel plays a key role in the regulation of carbohydrate metabolism, few attempts have been made to purify the enzyme (4, 17, 18) since the subject of P-fructokinase was last reviewed (19). The apparent instability of the enzyme makes purification difficult, and a number of suggestions have been offered to explain this pro...
متن کاملPurification and properties of sheep liver phosphofructokinase.
1. The activity of phosphofructokinase in sheep liver was found to be dependent on the composition and molarity of the buffer used in extraction. Under optimum conditions a value of 4-7mumoles/min./g. wet wt. of tissue was obtained. 2. The enzyme was purified 480-fold by a combination of ammonium sulphate fractionation, heat treatment in the presence of ethanol, DEAE-cellulose chromatography an...
متن کاملextraction and acetylation of purified trypsin from bovin pancreas and study of some its physico-chemical properties
آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...
15 صفحه اولStudies on structure of human erythrocyte phosphofructokinase.
Human erythrocyte phosphofructokinase has been purified to homogeneity and some of its physicochemical properties have been investigated. The enzyme exists in several polymeric states. At high protein concentrations (3 mglml), the predominant form of the enzyme shows a sedimentation coefficient of 57 S. In highly dilute solution (0.3 to 10 pg/ ml), the enzyme is in 12 S to 18 S forms as shown b...
متن کاملPurification and properties of human erythrocyte carbonic anhydrases.
Three different methods are described for separating hemoglobins from carbonic anhydrases in hemolysates from human erythrocytes. The preferred method involves adsorption on diethylaminoethyl Sephadex at pH 8.7 followed by selective elution of the carbonic anhydrases. Carbonic anhydrases A, B, and C are subsequently separated from each other on DEAE-Sephadex by elution with 0.05 M Trischloride ...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Enzymology
سال: 1972
ISSN: 0005-2744
DOI: 10.1016/0005-2744(72)90013-7