HSV2 acute retinal necrosis: diagnosis and monitoring with quantitative polymerase chain reaction

3214 Association of herpetic encephalitis with acute retinal necrosis syndrome proved by the polymerase chain reaction

Purpose : To discuss the definition and to determine the most logical intervention in treating patients with clinically foscamet resistant cytomgalovirus retinitis. Patients : Two patients with foscarnet clinically resistant cytomegalovims retinitis were followed in our department since june 1994. In these 2 patients, foscarnet was the first antiviral drug theraw civen for the treamxnt of cvtom...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Polymerase Chain Reaction for the Diagnosis of Tuberculosis

Dear Editor-in-Chief We read with interest the study by Khazaei et al. (1) in which the authors have nicely concluded that PCR is more sensitive test than Ziehl-Neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (TB). They have rightly pointed to use PCR, selectively, in acidfast bacilli negative paucibacillary forms of TB. However, we intend to highlight few...

Diagnosis of tubercular uveitis by quantitative polymerase chain reaction

PURPOSE To report the use of real-time or quantitative polymerase chain reaction (qPCR) in confirming the diagnosis of tubercular uveitis. METHODS A qPCR assay using primers targeting the MPB64 gene of Mycobacterium tuberculosis (MTB) was developed. A standard curve for quantification of mycobacterial load was generated. Vitreous fluid samples from patients presumed to have tubercular uveitis...

Diagnosis of bacteremia in patients with suspected septicemia using polymerase chain reaction method

Background: Sepsis or blood stream infection is a clinical lethal syndrome with severe systemic inflammatory response to infection, if not treated quickly, is associated with dangerous consequences and high morbidity and mortality. The traditional and conventional method for identification of sepsis is blood culture method which is so time-consuming and long that it eliminates the possibility o...