HPLC fingerprint of flavonoids, enzyme inhibition and antioxidant activity of Newbouldia laevis stem-bark: an in vitro and in silico study

نویسندگان

چکیده

Abstract Background Medicinal plant contains multiple bioactive compounds with therapeutic potentials. Due to their availability, affordability, and minimal known side effects, they are widely practiced. Identification, quantification, establishment of interaction physiological enzymes help in the standardization plant-based medicinal extracts. In this study, gas chromatography/flame ionization detector (GC–FID) high-performance liquid chromatography (HPLC) analysis were used determine components ethanol extract Newbouldia laevis stem bark. The antioxidant activity was determined. Enzyme inhibitory potency flavonoids’ investigated against acetylcholinesterase, butyrylcholinesterase, phospholipase A2, α-glucosidase, α-amylase. Results Analysis N. stem-bark revealed alkaloids (0.37%), tannins (1.82 mg/TEq/g), flavonoids (5.85 mg/QEq), steroids (0.11 mg/10 g) glycosides (0.08 g). HPLC fingerprint showed high concentrations (mg/100 catechin (47.11), apigenin (15.68), luteolin (18.90), kaempferol (41.54), quercetin (37.64), respectively. vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging ability exhibited at 150 200 mg/mL, At 300 mg, most potentials (lipid peroxidation, metal chelating ability, hydroxyl, nitric oxide, sulfide oxide radicals abilities) obtained. varying abilities (> 50%) on α-glucosidase α-amylase IC 50 129.46, 237.10, 169.50, 251.04 243.06 respectively, inhibition constants (Ki) 3.92, 1.63, 1.11, 2.95 2.11. an affinity for targeted free energies higher than standard drugs. Conclusion results that bark possesses enzyme has been implicated diabetes. silico these by suggests can be effective ameliorating complications associated diabetes mellitus.

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ژورنال

عنوان ژورنال: Future Journal of Pharmaceutical Sciences

سال: 2023

ISSN: ['2314-7245', '2314-7253']

DOI: https://doi.org/10.1186/s43094-023-00486-0