Honokiol induces superoxide production by targeting mitochondrial respiratory chain complex I in Candida albicans

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Honokiol induces superoxide production by targeting mitochondrial respiratory chain complex I in Candida albicans

BACKGROUND Honokiol, a compound extracted from Magnolia officinalis, has antifungal activities by inducing mitochondrial dysfunction and triggering apoptosis in Candida albicans. However, the mechanism of honokiol-induced oxidative stress is poorly understood. The present investigation was designed to determine the specific mitochondrial reactive oxygen species (ROS)-generation component. MET...

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Superoxide production by the mitochondrial respiratory chain.

Most of the oxygen consumed by aerobic organisms is reduced to water by the enzyme cytochrome c oxidase in the terminal reaction of the mitochondrial respiratory chain. Since oxygen in the ground state is in a triplet configuration (with two unpaired electrons in the outer shell) its reduction to water must occur in four consecutive one-electron steps. Some of the partially reduced oxygen inter...

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Honokiol induces reactive oxygen species-mediated apoptosis in Candida albicans through mitochondrial dysfunction

OBJECTIVE To investigate the effects of honokiol on induction of reactive oxygen species (ROS), antioxidant defense systems, mitochondrial dysfunction, and apoptosis in Candida albicans. METHODS To measure ROS accumulation, 2',7'-dichlorofluorescein diacetate fluorescence was used. Lipid peroxidation was assessed using both fluorescence staining and a thiobarbituric acid reactive substances (...

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Nonylphenol ethoxylate plastic additives inhibit mitochondrial respiratory chain complex I.

The first and major entry point of electrons into the mitochondrial respiratory chain (MRC) 1 occurs through NADH-coenzyme Q reduc-tase (complex I). Decreased complex I activity is associated with a wide range of conditions, including inherited mitochondrial diseases and neu-rodegenerative conditions such as Parkinson disease (1–2). More than 60 natural and commercial compounds are reported to ...

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The Assembly Pathway of Mitochondrial Respiratory Chain Complex I.

Mitochondrial complex I is the largest integral membrane enzyme of the respiratory chain and consists of 44 different subunits encoded in the mitochondrial and nuclear genome. Its biosynthesis is a highly complicated and multifaceted process involving at least 14 additional assembly factors. How these subunits assemble into a functional complex I and where the assembly factors come into play is...

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ژورنال

عنوان ژورنال: PLOS ONE

سال: 2017

ISSN: 1932-6203

DOI: 10.1371/journal.pone.0184003