Homologous pairing proteins encoded by the Escherichia coii recE and recT genes
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چکیده
منابع مشابه
Restriction-stimulated homologous recombination of plasmids by the RecE pathway of Escherichia coli.
To test the double-strand break (DSB) repair model in recombination by the RecE pathway of Escherichia coli, we constructed chimeric phages that allow restriction-mediated release of linear plasmid substrates of the bioluminescence recombination assay in infected EcoRI+ cells. Kinetics of DSB repair and expression of recombination products were followed by Southern hybridization and by the biol...
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PRINCIPLES OF HOMOLOGOUS PAIRING AND DNA STRAND EXCHANGE . Homologous Pairing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ... . . . . . . . DNA Strand Exchange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Energetic Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Experimental Assays . . . . . . . . . . . . . . . . . ...
متن کاملHomologous pairing and the role of pairing centers in meiosis.
Homologous pairing establishes the foundation for accurate reductional segregation during meiosis I in sexual organisms. This Commentary summarizes recent progress in our understanding of homologous pairing in meiosis, and will focus on the characteristics and mechanisms of specialized chromosome sites, called pairing centers (PCs), in Caenorhabditis elegans and Drosophila melanogaster. In C. e...
متن کاملRecombineering using RecTE from Pseudomonas syringae.
In this report, we describe the identification of functions that promote genomic recombination of linear DNA introduced into Pseudomonas cells by electroporation. The genes encoding these functions were identified in Pseudomonas syringae pv. syringae B728a based on similarity to the lambda Red Exo/Beta and RecET proteins encoded by the lambda and Rac bacteriophages of Escherichia coli. The abil...
متن کاملRole of superhelicity in homologous pairing of DNA molecules promoted by Escherichia coli recA protein.
In the presence of ATP and an excess of recA protein, superhelical closed circular DNA (form I DNA) and homologous single-stranded fragments paired to form D-loops in the early stage of incubation and dissociated during subsequent incubation. RecA protein that was not bound to single-stranded DNA ("free recA protein") was shown to be responsible for the dissociation of D-loops. Larger amount of...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 1994
ISSN: 0950-382X,1365-2958
DOI: 10.1111/j.1365-2958.1994.tb00286.x