منابع مشابه
Nuclear Membranes of Cultured Mammalian Cells
When kidney cells are cultured directly from the rabbit, the nuclear membranes undergo a change that can be measured as an increase in electrophoretic mobility. The change appears to begin immediately upon culture and is maximal 2 hours later, after which the mobility remains constant at the elevated level. Actinomycin D and p-fluorophenylalanine, but not EDTA or ionizing radiation, suppress th...
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Although understanding cells' responses to mechanical stimuli is seen as increasingly important for understanding cell biology, how to best measure, interpret, and model cells' mechanical properties remains unclear. We determine the frequency-dependent shear modulus of cultured mammalian cells by using four different methods, both unique and well established. This approach clarifies the effects...
متن کاملAutofluorescence of viable cultured mammalian cells.
The autofluorescence other than intrinsic protein emission of viable cultured mammalian cells has been investigated. The fluorescence was found to originate in discrete cytoplasmic vesicle-like regions and to be absent from the nucleus. Excitation and emission spectra of viable cells revealed at least two distinct fluorescent species. Comparison of cell spectra with spectra of known cellular me...
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Bovine neurotubulin has been labeled with dichlorotriazinyl-aminofluorescein (DTAF-tubulin) and microinjected into cultured mammalian cells strains PTK1 and BSC. The fibrous, fluorescence patterns that developed in the microinjected cells were almost indistinguishable from the pattern of microtubules seen in the same cells by indirect immunofluorescence. DTAF-tubulin participated in the formati...
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Experimental studies on the complex process of mutagenesis are conducted in the belief that they will increase our understanding of the effects of environmental agents on human populations. Cultured mammalian cells offer distinct advantages over other biological systems for studying this problem. Mutagenesis in cultured mammalian cells is unquestionably more relevant to man than are bacterial, ...
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ژورنال
عنوان ژورنال: The Scientific World JOURNAL
سال: 2002
ISSN: 1537-744X
DOI: 10.1100/tsw.2002.848