Holographic virtual staining of individual biological cells

Quantitative Analysis of Biological Cells Using Digital Holographic Microscopy

Holographic characterization of individual colloidal spheres’ porosities

Synthetic colloidal spheres play a central role in applications as diverse as catalysis, medical diagnostics, and photonics. Even as many applications advance toward single-sphere implementations, methods for characterizing colloidal spheres typically offer only sampleaveraged overviews of such properties as spheres’ sizes and porosities. Moreover, such characterization methods as mercury adsor...

Biological imaging by Digital Holographic Adaptive Optics

The basic principles of DHAO have been presented and demonstrated in a recent paper [Opt. Lett. 36, 27102712(2011)]. In this paper, the application of DHAO in biological imaging is investigated. OCIS codes: 090.1995; 110.1080

Characterizations of individual mouse red blood cells parasitized by Babesia microti using 3-D holographic microscopy

Babesia microti causes "emergency" human babesiosis. However, little is known about the alterations in B. microti invaded red blood cells (Bm-RBCs) at the individual cell level. Through quantitative phase imaging techniques based on laser interferometry, we present the simultaneous measurements of structural, chemical, and mechanical modifications in individual mouse Bm-RBCs. 3-D refractive ind...

Targeted alteration of real and imaginary refractive index of biological cells by histological staining.

Various staining techniques are commonly used in biomedical research to investigate cellular morphology. By inducing absorption of light, staining dyes change the intracellular refractive index due to the Kramers-Kronig relationship. We present a method for creating 2D maps of real and imaginary refractive indices of stained biological cells using their thickness and absorptance. We validate ou...