Holistic protocol for callus culture optimization using statistical modelling

Statistically optimized biotransformation protocol for continuous production of L-DOPA using Mucuna monosperma callus culture

L-DOPA (3,4-dihydroxyphenyl-L-alanine), a modified amino acid, is an expansively used drug for the Parkinson's disease treatment. In the present study, optimization of nutritional parameters influencing L-DOPA production was attempted using the response surface methodology (RSM) from Mucuna monosperma callus. Optimization of the four factors was carried out using the Box-Behnken design. The opt...

Callus culture and regeneration.

Efficient protocol for break impasses of regeneration via callus for 20 genotypes of chickpea

The results of studies on in vitro regeneration of chickpea (Cicer arietinum L.) have shown that indirect regeneration via callus was not successful. The introduction of an efficient and repeatable regeneration protocol is necessary for using transgenic technologies for the improvement of chickpea. The present study was undertaken to investigate the possibility of indirect regeneration in vario...

Statistical Optimization of Conditions for Maximize Production of Mannan by Saccharomyces Cerevisiae Using Response Surface Methodology

In view of the increase in Saccharomyces cerevisiae mannan content, the culture condition for S.cerevisiae were optimized in this study. The influence of culture condition such as original pH, inoculum size, and temperature on mannan production were evaluated using Response surface methodology. The mathematical model was established by the quadratic rotary combination design. with the order of ...

Production modelling for holistic production control

1569-190X/$ see front matter 2012 Elsevier B.V http://dx.doi.org/10.1016/j.simpat.2012.07.010 ⇑ Corresponding author. Tel.: +386 1 477 36 84. E-mail address: [email protected] (M. Glavan). Holistic production control is a concept that introduces production optimisation by employing model-based, closed-loop control of the principal production Performance Indicators (pPIs). The concept relies on...